Peroxisome proliferator-activated receptor gamma (pparg), Xenopus laevis, recombinant

Organism: Xenopus laevis (African clawed frog). Source: E.coli. Expression Region: 1-477aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MVDTEMPFWS NLNFGMNSMD MSALEDHCQP YDIKPFTTVD FSSINSHYDD ILDEKTFLCR NDQSPIDYKY DLKLQECQSS IKLEPPSPPY FSDKPQCSKA FEDTPNSFIA IECRVCGDKA SGFHYGVHAC EGCKGFFRRT IRLKLIYERC DLNCRIHKKS RNKCQFCRFQ KCLAVGMSHN AIRFGRMPQA EKEKLLAEIS SDIDQLNPES ADQRVLAKHL YDSYVKSFPL TKAKAPGHPD GQSHRQNSRG YTRHELADDG GGSDQGAVRE PRAEQGGGDS NLPALSVALR GGVREITEFA KNIPGFVSLD LNDQVTLLKY GVHEIIFTML ASLMNKDGVL VAEGQGFMTR EFLKSLRKPF SDFMEPKFEF AIRFNSLELD DSDLAIFVAV IILSGDRPGL LNVKPIEDIQ DSLLQALELQ LKLNHPDSAQ LFAKLLQKMT DLRQVVTEHV QLLQLIKKTE ADMCLHPLLQ EIYKDLY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. May play a role in the regulation of circadian rhythm . Reference: "Xenopus peroxisome proliferator activated receptors: genomic organization, response element recognition, heterodimer formation with retinoid X receptor and activation by fatty acids." Krey G., Keller H., Mahfoudi A., Medin J., Ozato K., Dreyer C., Wahli W. J. Steroid Biochem. Mol. Biol. 47:65-73(1993). Function: nan