P-selectin (SELP), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 42-771aa. Protein Length: Partial. Tag Info: C-terminal hFc-Myc-tagged. Target Protein Sequence: WTYHYSTKAY SWNISRKYCQ NRYTDLVAIQ NKNEIDYLNK VLPYYSSYYW IGIRKNNKTW TWVGTKKALT NEAENWADNE PNNKRNNEDC VEIYIKSPSA PGKWNDEHCL KKKHALCYTA SCQDMSCSKQ GECLETIGNY TCSCYPGFYG PECEYVRECG ELELPQHVLM NCSHPLGNFS FNSQCSFHCT DGYQVNGPSK LECLASGIWT NKPPQCLAAQ CPPLKIPERG NMTCLHSAKA FQHQSSCSFS CEEGFALVGP EVVQCTASGV WTAPAPVCKA VQCQHLEAPS EGTMDCVHPL TAFAYGSSCK FECQPGYRVR GLDMLRCIDS GHWSAPLPTC EAISCEPLES PVHGSMDCSP SLRAFQYDTN CSFRCAEGFM LRGADIVRCD NLGQWTAPAP VCQALQCQDL PVPNEARVNC SHPFGAFRYQ SVCSFTCNEG LLLVGASVLQ CLATGNWNSV PPECQAIPCT PLLSPQNGTM TCVQPLGSSS YKSTCQFICD EGYSLSGPER LDCTRSGRWT DSPPMCEAIK CPELFAPEQG SLDCSDTRGE FNVGSTCHFS CDNGFKLEGP NNVECTTSGR WSATPPTCKG IASLPTPGLQ CPALTTPGQG TMYCRHHPGT FGFNTTCYFG CNAGFTLIGD STLSCRPSGQ WTAVTPACRA VKCSELHVNK PIAMNCSNLW GNFSYGSICS FHCLEGQLLN GSAQTACQEN GHWSTTVPTC QAGPLTIQEA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Reference: "Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force." Mehta-D'souza P., Klopocki A.G., Oganesyan V., Terzyan S., Mather T., Li Z., Panicker S.R., Zhu C., McEver R.P. J. Biol. Chem. 292:2510-2518(2017). Function: nan