Nucleobindin-2 (NUCB2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 25-420aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD ELKRQEVGRL RMLIKAKLDS LQDIGMDHQA LLKQFDHLNH LNPDKFESTD LDMLIKAATS DLEHYDKTRH EEFKKYEMMK EHERREYLKT LNEEKRKEEE SKFEEMKKKH ENHPKVNHPG SKDQLKEVWE ETDGLDPNDF DPKTFFKLHD VNSDGFLDEQ ELEALFTKEL EKVYDPKNEE DDMVEMEEER LRMREHVMNE VDTNKDRLVT LEEFLKATEK KEFLEPDSWE TLDQQQFFTE EELKEYENII ALQENELKKK ADELQKQKEE LQRQHDQLEA QKLEYHQVIQ QMEQKKLQQG IPPSGPAGEL KFEPHI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium-binding protein which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3. , [Nesfatin-1]: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Reference: n/a. Function: nan