Nicotinamide N-Methyltransferase (human, recombinant)

Nicotinamide N-Methyltransferase (NNMT) methylates nicotinamide and other pyridine containing compounds. NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nitrogen N1 of nicotinamide to produce 1-methylnicotinamide (MNA) and S-adenosylhomocysteine. MNA is a messenger molecule that increases neurite branching, serves as an anti-thrombotic, and has anti-inflammatory properties. The role of NNMT overexpression in cancers may be to alter epigenetic methylation patterns in two ways: by lowering the intracellular concentration of SAM, required by methyltransferase enzymes, and by depleting the available NAD+ by transforming nicotinamide to MNA. Sirtuins use NAD+ as a substrate to alter protein acetylation and ribosylation, including histone targets.Synonyms: NNMT. Purity: >95% estimated by SDS-PAGE. Source: Recombinant N-terminal His- and SUMO-tagged protein expressed in E. coli. Amino Acids: 2-264 (full length). MW: 42.6 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 8.0, containing 500 mM sodium chloride, 5% glycerol, and 1 mM DTT. License: SUMOpro tag was used under non-exclusive license from LifeSensors, Inc.. UniProt Accession #: P40261.