Neurofibromin (NF1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1566-1837aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SSKFEEFMTR HQVHEKEEFK ALKTLSIFYQ AGTSKAGNPI FYYVARRFKT GQINGDLLIY HVLLTLKPYY AKPYEIVVDL THTGPSNRFK TDFLSKWFVV FPGFAYDNVS AVYIYNCNSW VREYTKYHER LLTGLKGSKR LVFIDCPGKL AEHIEHEQQK LPAATLALEE DLKVFHNALK LAHKDTKVSI KVGSTAVQVT SAERTKVLGQ SVFLNDIYYA SEIEEICLVD ENQFTLTIAN QGTPLTFMHQ ECEAIVQSII HIRTRWELSQ PD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. Reference: "A conserved alternative splice in the von Recklinghausen neurofibromatosis (NF1) gene produces two neurofibromin isoforms, both of which have GTPase-activating protein activity." Andersen L.B., Ballester R., Marchuk D.A., Chang E., Gutmann D.H., Saulino A.M., Camonis J., Wigler M., Collins F.S. Mol. Cell. Biol. 13:487-495(1993). Function: nan