NAD-dependent protein deacetylase sirtuin-1 (SIRT1), recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 2-747aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: ADEAALALQP GGSPSAAGAD REAASSPAGE PLRKRPRRDG PGLERSPGEP GGAAPEREVP AAARGCPGAA AAALWREAEA EAAAAGGEQE AQATAAAGEG DNGPGLQGPS REPPLADNLY DEDDDDEGEE EEEAAAAAIG YRDNLLFGDE IITNGFHSCE SDEEDRASHA SSSDWTPRPR IGPYTFVQQH LMIGTDPRTI LKDLLPETIP PPELDDMTLW QIVINILSEP PKRKKRKDIN TIEDAVKLLQ ECKKIIVLTG AGVSVSCGIP DFRSRDGIYA RLAVDFPDLP DPQAMFDIEY FRKDPRPFFK FAKEIYPGQF QPSLCHKFIA LSDKEGKLLR NYTQNIDTLE QVAGIQRIIQ CHGSFATASC LICKYKVDCE AVRGDIFNQV VPRCPRCPAD EPLAIMKPEI VFFGENLPEQ FHRAMKYDKD EVDLLIVIGS SLKVRPVALI PSSIPHEVPQ ILINREPLPH LHFDVELLGD CDVIINELCH RLGGEYAKLC CNPVKLSEIT EKPPRTQKEL AYLSELPPTP LHVSEDSSSP ERTSPPDSSV IVTLLDQAAK SNDDLDVSES KGCMEEKPQE VQTSRNVESI AEQMENPDLK NVGSSTGEKN ERTSVAGTVR KCWPNRVAKE QISRRLDGNQ YLFLPPNRYI FHGAEVYSDS EDDVLSSSSC GSNSDSGTCQ SPSLEEPMED ESEIEEFYNG LEDEPDVPER AGGAGFGTDG DDQEAINEAI SVKQEVTDMN YPSNKS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). MAPK8/JNK1 and RPS19BP1/AROS act as positive regulators of deacetylation activity. Negatively regulated by CCAR2. Reference: n/a. Function: nan