N (G),N (G)-dimethylarginine dimethylaminohydrolase 1 (DDAH1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-285aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AGLGHPAAFG RATHAVVRAL PESLGQHALR SAKGEEVDVA RAERQHQLYV GVLGSKLGLQ VVELPADESL PDCVFVEDVA VVCEETALIT RPGAPSRRKE VDMMKEALEK LQLNIVEMKD ENATLDGGDV LFTGREFFVG LSKRTNQRGA EILADTFKDY AVSTVPVADG LHLKSFCSMA GPNLIAIGSS ESAQKALKIM QQMSDHRYDK LTVPDDIAAN CIYLNIPNKG HVLLHRTPEE YPESAKVYEK LKDHMLIPVS MSELEKVDGL LTCCSVLINK KVDS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes N,N-dimethyl-L-arginine and N-monomethyl-L-arginine which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Reference: "Mechanism of 4-HNE mediated inhibition of hDDAH-1: implications in NO regulation." Forbes S.P., Druhan L.J., Guzman J.E., Parinandi N., Zhang L., Green-Church K.B., Cardounel A.J. Biochemistry 47:1819-1826(2008). Function: nan