mFluor(TM) Yellow 630-streptavidin conjugate

Ex: 611 nm. Em: 630 nm. Soluble in: Water. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluorT Yellow 630-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluorT Yellow 610 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 561 nm excitation (e.g., the Violet Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.