Membrane cofactor protein (CD46), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 164-325aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: PPPKIKNGKH TFSEVEVFEY LDAVTYSCDP APGPDPFSLI GESTIYCGDN SVWSRAAPEC KVVKCRFPVV ENGKQISGFG KKFYYKATVM FECDKGFYLD GSDTIVCDSN STWDPPVPKC LKVSTSSTTK SPASSASGPR PTYKPPVSNY PGYPKPEEGI LDKGRIVTDR SASRVSVRT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells., (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. (Microbial infection) Acts as a receptor for cultured Measles virus. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes . Reference: "Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype." Kemper C., Chan A.C., Green J.M., Brett K.A., Murphy K.M., Atkinson J.P. Nature 421:388-392(2003). Function: nan