Major prion protein (PRNP), Mesocricetus auratus, recombinant

Organism: Mesocricetus auratus (Golden hamster). Source: E.coli. Expression Region: 23-231aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: KKRPKPGGWN TGGSRYPGQG SPGGNRYPPQ GGGTWGQPHG GGWGQPHGGG WGQPHGGGWG QPHGGGWGQG GGTHNQWNKP SKPKTNMKHM AGAAAAGAVV GGLGGYMLGS AMSRPMMHFG NDWEDRYYRE NMNRYPNQVY YRPVDQYNNQ NNFVHDCVNI TIKQHTVTTT TKGENFTETD IKIMERVVEQ MCTTQYQKES QAYYDGRRS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. Reference: "Purification and properties of the cellular and scrapie hamster prion proteins." Turk E., Teplow D.B., Hood L.E., Prusiner S.B. Eur. J. Biochem. 176:21-30(1988). Function: nan