Lymphocyte activation gene 3 protein (Lag3), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 24-442aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: GPGKELPVVW AQEGAPVHLP CSLKSPNLDP NFLRRGGVIW QHQPDSGQPT PIPALDLHQG MPSPRQPAPG RYTVLSVAPG GLRSGRQPLH PHVQLEERGL QRGDFSLWLR PALRTDAGEY HATVRLPNRA LSCSLRLRVG QASMIASPSG VLKLSDWVLL NCSFSRPDRP VSVHWFQGQN RVPVYNSPRH FLAETFLLLP QVSPLDSGTW GCVLTYRDGF NVSITYNLKV LGLEPVAPLT VYAAEGSRVE LPCHLPPGVG TPSLLIAKWT PPGGGPELPV AGKSGNFTLH LEAVGLAQAG TYTCSIHLQG QQLNATVTLA VITVTPKSFG LPGSRGKLLC EVTPASGKER FVWRPLNNLS RSCPGPVLEI QEARLLAERW QCQLYEGQRL LGATVYAAES SSGAHSARRI SGDLKGGHL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II), the precise role of MHC-II-binding is however unclear. , [Secreted lymphocyte activation gene 3 protein]: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. Reference: n/a. Function: nan