LBH-589 (Panobinostat, NVP-LBH589)

Panobinostat, a histone deacetylase inhibitor, is being tested in humans against cutaneous T cell lymphoma, prostate cancer, myelodysplastic syndromes, breast cancer, chronic myelogenous leukemia, and other types of malignant disease. Panobinostat inhibited histone deacetylase and induced acetylation of histone H3 and alpha-tubulin protein in human umbilical vein endothelial cells (HUVEC), which resulted in induction of G(2)-M cell cycle arrest and inhibition of HUVEC proliferation and viability. Panobinostat at noncytotoxic concentrations inhibited endothelial tube formation, Matrigel invasion, AKT activity, extracellular signal-regulated kinase 1/2 phosphorylation and chemokine receptor CXCR4 expression. It aslo reduced angiogenesis and PC-3 tumor growth in mice. Synonyms: (2E)-N-Hydroxy-3-[4-[[[2-(2-methyl-1H-indol-3-yl)ethyl]amino]methyl]phenyl]-2-propenamide, LBH 589, LBH-589, CAS No: 404950-80-7, Molecular Formula: C??H??N?O?, Molecular Weight: 349.43, Appearance: Supplied as a white to off-white powder. Purity: >99%, Solubility: Soluble in DMSO at 200mg/ml, soluble in ethanol at 5mg/ml with warming, very poorly soluble in water, maximum solubility in plain water is estimated to be about 20-50uM, buffers, serum or other additives may increase or decrease the aqueous solubility. Elemental Anaylsis: Calculated: , C=72.18%, H=6.63%, N=12.03%, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards:  All products should be handled by qualified personnel only, trained in laboratory procedures.