Lanosterol 14-alpha-demethylase (human, recombinant)

Lanosterol 14-alpha-demethylase, also known as cytochrome P450 (CYP) isoform CYP51A1, is a heme-containing demethylase and member of the CYP450 superfamily that demethylates lanosterol (Cay-19521) to generate sterol precursors in the synthesis of cholesterol (Cay-39088, Cay-9003100). It is ubiquitously expressed with higher levels found in the testes and ovaries and has a globular structure that is composed of a hydrophobic substrate channel, a substrate-binding cavity, and a heme-iron cofactor conjugation region. Inhibition of lanosterol 14-alpha-demethylase decreases cholesterol levels and induces differentiation in HL-60 promyeoloblast leukemia cells. Cayman's Lanosterol 14-alpha-demethylase (Cay-32928) protein can be used for ELISA, enzyme activity assay, and Western blot applications.Synonyms: Cytochrome P450 51A1, Cytochrome P45014DM, Cytochrome P450LI, CYP51A1, CYP51, LDM. Purity: >60% estimated by SDS-PAGE. Source: Active recombinant human C-terminal His-tagged lanosterol 14-alpha-demethylase expressed in E. coli. Amino Acids: 61-503. MW: 52.71 kDa. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.6, with 0.5 mM EDTA, 0.2 mM DTT, and 10% glycerol. UniProt Accession #: Q16850.