JAK2 JH1 and JH2 Domains (human, recombinant, aa 532-1,132)

JAK2 is a non-receptor tyrosine kinase that has roles in immune signaling.1,2 It is composed of N-terminal FERM and SH2 domains, a regulatory JH2 pseudokinase domain, and a C-terminal JH1 kinase domain. It is widely expressed and associates with class I and class II cytokine receptors at the plasma membrane.3,4 Activation of these cytokine receptors activates JAK2 and induces its dimerization and kinase activity, leading to JAK2 phosphorylation of STAT transcription factors and transcription of immune-related target genes. JAK2 signaling is inhibited by the suppressor of cytokine signaling (SOCS) proteins SOCS1 and SOCS3.5,6 Gain-of-function mutations in JAK2 are associated with various blood disorders, including leukemias and myeloproliferative neoplasms.4 Cayman's JAK2 JH1 and JH2 Domains (human, recombinant, aa 532-1,132) protein can be used for enzyme activity assays and has a calculated molecular weight of 98 kDa. WARNING This product is not for human or veterinary use. Purity: >80% estimated by SDS-PAGE. Source: Active recombinant human N-terminal His- and GST-tagged JAK2 JH1 and JH2 domains expressed in insect cells. Amino Acids: 532-1,132. MW: 98 kDa. Formulation: 45 mM Tris-HCl, pH 8.0, with 300 mM sodium chloride, 2.4 mM potassium chloride, 0.04% polysorbate 20, 3 mM DTT, 10% glycerol, and variable imidazole.