IRAK4 (human, recombinant)

IL-1 receptor-associated kinase 4 (IRAK4) is a serine/threonine kinase involved in Toll/IL-1 receptor (TIR) signaling. It is composed of an N-terminal death domain, which is involved in dimerization and interaction with MyD88, a proline/serine/threonine-rich domain, and a C-terminal kinase domain. IRAK4 is ubiquitously expressed and localizes to the cytoplasm. It has roles in innate and adaptive immunity, interacts with IRAK1, MyD88, and TNF receptor-associated factor 6 (TRAF6), and signals through the NF-kappaB and MAPK pathways. Homozygous knockout of Irak4 increases susceptibility to M. bovis infection in mice and decreases survival and increases lung bacterial burden in a mouse model of M. tuberculosis infection, however, Irak4-/- mice are resistant to LPS-induced septic shock. Mutations in IRAK4 are associated with an increased risk of invasive bacterial infections in children. Cayman's IRAK4 (human, recombinant) protein can be used for enzyme activity assays. This protein has a calculated molecular weight of 34.4 kDa.Synonyms: IL-1 Receptor-associated Kinase 4, Interleukin-1 Receptor-associated Kinase 4, Renal Carcinoma Antigen NY-REN-64. Purity: >90% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant human IRAK4 expressed in insect cells. Amino Acids: 154-460. MW: 34.4 kDa. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol. UniProt Accession No: Q9NWZ3.