Intestinal-type alkaline phosphatase (ALPI), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 20-503aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VIPAEEENPA FWNRQAAEAL DAAKKLQPIQ KVAKNLILFL GDGLGVPTVT ATRILKGQKN GKLGPETPLA MDRFPYLALS KTYNVDRQVP DSAATATAYL CGVKANFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKQAG KSVGVVTTTR VQHASPAGTY AHTVNRNWYS DADMPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFPM GTPDPEYPAD ASQNGIRLDG KNLVQEWLAK HQGAWYVWNR TELMQASLDQ SVTHLMGLFE PGDTKYEIHR DPTLDPSLME MTEAALRLLS RNPRGFYLFV EGGRIDHGHH EGVAYQALTE AVMFDDAIER AGQLTSEEDT LTLVTADHSH VFSFGGYTLR GSSIFGLAPS KAQDSKAYTS ILYGNGPGYV FNSGVRPDVN ESESGSPDYQ QQAAVPLSSE THGGEDVAVF ARGPQAHLVH GVQEQSFVAH VMAFAACLEP YTACDLAPPA CTTD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Miscellaneous In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney). Catalytic activity a phosphate monoester + H2O = an alcohol + phosphate. Reference: "Structural evidence of functional divergence in human alkaline phosphatases." Le Du M.H., Millan J.L. J. Biol. Chem. 277:49808-49814(2002). Function: nan