Intestinal-type alkaline phosphatase (ALPI) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 20-503aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: VIPAEEENPA FWNRQAAEAL DAAKKLQPIQ KVAKNLILFL GDGLGVPTVT ATRILKGQKN GKLGPETPLA MDRFPYLALS KTYNVDRQVP DSAATATAYL CGVKANFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKQAG KSVGVVTTTR VQHASPAGTY AHTVNRNWYS DADMPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFPM GTPDPEYPAD ASQNGIRLDG KNLVQEWLAK HQGAWYVWNR TELMQASLDQ SVTHLMGLFE PGDTKYEIHR DPTLDPSLME MTEAALRLLS RNPRGFYLFV EGGRIDHGHH EGVAYQALTE AVMFDDAIER AGQLTSEEDT LTLVTADHSH VFSFGGYTLR GSSIFGLAPS KAQDSKAYTS ILYGNGPGYV FNSGVRPDVN ESESGSPDYQ QQAAVPLSSE THGGEDVAVF ARGPQAHLVH GVQEQSFVAH VMAFAACLEP YTACDLAPPA CTTD. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate (pNPP), in 1 minute at 37°C, pH10.0. The specific activity is > 8836.463 U/mg. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Alkaline phosphatase that can hydrolyze various phosphate compounds. Reference: n/a. Function: nan