Interleukin-2 receptor subunit alpha (IL2RA), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 22-213aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SC. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: ?Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 µg/mL can bind Anti-IL2RA recombinant antibody (CSB-RA011649MA1HU) , the EC50 is 2.463-3.353 ng/mL.?Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 µg/mL can bind Human IL2(CSB-MP011629HU) , the EC50 is 1.693-2.039 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involve in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. Reference: "Molecular cloning of cDNA encoding human interleukin-2 receptor." Nikaido T., Shimizu A., Ishida N., Sabe H., Teshigawara K., Maeda M., Uchiyama T., Yodoi J., Honjo T. Nature 311:631-635 (1984). Function: nan