Inducible ornithine decarboxylase (speF), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 1-732aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSKLKIAVSD SCPDCFTTQR ECIYINESRN IDVAAIVLSL NDVTCGKLDE IDATGYGIPV FIATENQERV PAEYLPRISG VFENCESRRE FYGRQLETAA SHYETQLRPP FFRALVDYVN QGNSAFDCPG HQGGEFFRRH PAGNQFVEYF GEALFRADLC NADVAMGDLL IHEGAPCIAQ QHAAKVFNAD KTYFVLNGTS SSNKVVLNAL LTPGDLVLFD RNNHKSNHHG ALLQAGATPV YLETARNPYG FIGGIDAHCF EESYLRELIA EVAPQRAKEA RPFRLAVIQL GTYDGTIYNA RQVVDKIGHL CDYILFDSAW VGYEQFIPMM ADCSPLLLDL NENDPGILVT QSVHKQQAGF SQTSQIHKKD SHIKGQQRYV PHKRMNNAFM MHASTSPFYP LFAALNINAK MHEGVSGRNM WMDCVVNGIN ARKLILDNCQ HIRPFVPELV DGKPWQSYET AQIAVDLRFF QFVPGEHWHS FEGYAENQYF VDPCKLLLTT PGIDARNGEY EAFGVPATIL ANFLRENGVV PEKCDLNSIL FLLTPAEDMA KLQQLVALLV RFEKLLESDA PLAEVLPSIY KQHEERYAGY TLRQLCQEMH DLYARHNVKQ LQKEMFRKEH FPRVSMNPQE ANYAYLRGEV ELVRLPDAEG RIAAEGALPY PPGVLCVVPG EIWGGAVLRY FSALEEGINL LPGFAPELQG VYIEEHDGRK QVWCYVIKPR DAQSTLLKGE KL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006). Function: nan