IDH1 (human recombinant)

Isocitrate dehydrogenases (IDHs) are NAD+ and NADP+-dependent enzymes that catalyze the third step of the tricarboxylic acid cycle. IDHs catalyze oxidative decarboxylation of isocitrate producing alpha-ketoglutarate and carbon dioxide. IDH1 (cytosolic) and IDH2 (mitochondrial) are NADP+-dependent enzymes that catalyze reversible reactions. The IDH3 isoform, a NAD+-dependent multisubunit enzyme, is irreversible and allosterically regulated by a variety of positive (calcium, ADP, and citrate) and negative (ATP, NADH, and NADPH) effectors. IDH1 and IDH2 are mutated in >70% of lower grade gliomas.Synonyms: Isocitrate Dehydrogenase (NADP) Cytoplasmic. Purity: >90% estimated by SDS-PAGE. Source: recombinant C-terminal His-tagged protein expressed in E. coli. MW: 47.9 kDa. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM beta-mercaptoethanol, 50 mM calcium chloride, and 20% glycerol. UniProt Accession #: O75874.