Hydroxymethylglutaryl-CoA synthase, mitochondrial (Hmgcs2), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 38-508aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: TIPPAPLAKT DTWPKDVGIL ALEVYFPAQY VDQTDLEKFN NVEAGKYTVG LGQTRMGFCS VQEDINSLCL TVVQRLMERT KLPWDAVGRL EVGTETIIDK SKAVKTVLME LFQDSGNTDI EGIDTTNACY GGTASLFNAA NWMESSYWDG RYALVVCGDI AVYPSGNARP TGGAGAVAML IGPKAPLVLE QGLRGTHMEN AYDFYKPNLA SEYPLVDGKL SIQCYLRALD RCYAAYRKKI QNQWKQAGNN QPFTLDDVQY MIFHTPFCKM VQKSLARLMF NDFLSSSSDK QNNLYKGLEA FRGLKLEETY TNKDVDKALL KASLDMFNQK TKASLYLSTN NGNMYTSSLY GCLASLLSHH SAQELAGSRI GAFSYGSGLA ASFFSFRVSK DASPGSPLEK LVSSVSDLPK RLDSRRRMSP EEFTEIMNQR EQFYHKVNFS PPGDTSNLFP GTWYLERVDE MHRRKYARCP V. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate. Reference: n/a. Function: nan