Heat-stable enterotoxin receptor (Gucy2c), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Baculovirus. Expression Region: 23-429aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SQVRQKCHNG TYEISVLMMD NSAYKEPLQN LRDAVEEGLD IVRKRLREAE LNVTVNATFI YSDGLIHKSG DCRSSTCEGL DLLREITRDR KMGCVLMGPS CTYSTFQMYL DTELNYPMIS AGSFGLSCDY KETLTRILPP ARKLMYFLVD FWKVNNAPFK TFSWNSSYVY KNGSEPEDCF WYLNALEAGV SYFSEVLSFK DVLRRSEQFQ EILMGRNRKS NVIVMCGTPE TFYNVKGDLK VADDTVVILV DLFSNHYFED DTRAPEYMDN VLVLTLPPEK FIANASVSGR FPSERSDFSL AYLEGTLLFG HMLQTFLENG ESVTTPKFAR AFRNLTFQGL EGPVTLDDSG DIDNIMCLLY VSLDTRKYKV LMAYDTHKNQ TIPVATSPNF IWKNHRLPND VPGLGPQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin. Reference: "Guanylyl cyclase is a heat-stable enterotoxin receptor." Schulz S., Green C.K., Yuen P.S.T., Garbers D.L. Cell 63:941-948(1990). Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.