Heat-stable enterotoxin receptor (GUCY2C), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 24-430aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: SQVSQNCHNG SYEISVLMMG NSAFAEPLKN LEDAVNEGLE IVRGRLQNAG LNVTVNATFM YSDGLIHNSG DCRSSTCEGL DLLRKISNAQ RMGCVLIGPS CTYSTFQMYL DTELSYPMIS AGSFGLSCDY KETLTRLMSP ARKLMYFLVN FWKTNDLPFK TYSWSTSYVY KNGTETEDCF WYLNALEASV SYFSHELGFK VVLRQDKEFQ DILMDHNRKS NVIIMCGGPE FLYKLKGDRA VAEDIVIILV DLFNDQYFED NVTAPDYMKN VLVLTLSPGN SLLNSSFSRN LSPTKRDFAL AYLNGILLFG HMLKIFLENG ENITTPKFAH AFRNLTFEGY DGPVTLDDWG DVDSTMVLLY TSVDTKKYKV LLTYDTHVNK TYPVDMSPTF TWKNSKLPND ITGRGPQ. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 µg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 11.89-24.13 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin. Reference: n/a. Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.