Heat shock protein HSP70 (HSP70), Toxoplasma gondii, recombinant

Organism: Toxoplasma gondii (strain ATCC 50611 / Me49). Source: E.coli. Expression Region: 1-674aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MADSPAVGID LGTTYSCVGV WKNDAVEIIA NDQGNRTTPS YVAFTDTERL VGDAAKNQVA RNPENTIFDA KRLIGRKFDD PSVQSDMKHW PFKVIAGPGD KPLIEVTYQG EKKTFHPEEV SAMVLGKMKE IAEAYLGKEV KEAVITVPAY FNDSQRQATK DAGTIAGLSV LRIINEPTAA AIAYGLDKKG CGEMNVLIFD MGGGTFDVSL LTIEDGIFEV KATAGDTHLG GEDFDNRLVD FCVQDFKRKN RGKDISTNSR ALRRLRTQCE RTKRTLSSST QATIEIDSLF EGIDYSVSIS RARFEELCMD YFRNSLLPVE KVLKDSGIDK RSVSEVVLVG GSTRIPKIQQ LITDFFNGKE PCRSINPDEA VAYGAAVQAA ILKGVTSSQV QDLLLLDVAP LSLGLETAGG VMTKLIERNT TIPTKKSQTF TTYADNQPGV LIQVYEGERA MTKDNNLLGK FHLDGIPPAP RGVPQIEVTF DIDANGIMNV TAQDKSTGKS NQITITNDKG RLSASEIDRM VQEAEKYKAE DEQNKHRVEA KNGLENYCYH MRQTLDDEKL KDKISSEDRD TANKAIQEAL DWLDKNQLAE KEEFEAKQKE VESVCTPIIT KLYQAGAAAG GMPGGMGGMP GGMGGMPGGM GGMPGGMGGM PGGMGGMPGA GMGGSGGPTV EEVD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: Sibley D., Venepally P., Karamycheva S., Hadjithomas M., Khan A., Brunk B., Roos D., Caler E., Lorenzi H. Submitted (APR-2013). Function: nan