Growth hormone receptor (GHR), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 27-264aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: AILSRAPWSL QSVNPGLKTN SSKEPKFTKC RSPERETFSC HWTDEVHHGT KNLGPIQLFY TRRNTQEWTQ EWKECPDYVS AGENSCYFNS SFTSIWIPYC IKLTSNGGTV DEKCFSVDEI VQPDPPIALN WTLLNVSLTG IHADIQVRWE APRNADIQKG WMVLEYELQY KEVNETKWKM MDPILTTSVP VYSLKVDKEY EVRVRSKQRN SGNYGEFSEV LYVTLPQMSQ FTCEEDFY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: ?Measured by its binding ability in a functional ELISA. Immobilized GH1 (CSB-MP009407HU) at 1 µg/ml can bind human GHR, the EC50 of human GHR protein is 24.96-33.39 ng/ml. ?Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. Reference: n/a. Function: nan