Glycine receptor subunit alpha-1 (GLRA1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-250aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ARSAPKPMSP SDFLDKLMGR TSGYDARIRP NFKGPPVNVS CNIFINSFGS IAETTMDYRV NIFLRQQWND PRLAYNEYPD DSLDLDPSML DSIWKPDLFF ANEKGAHFHE ITTDNKLLRI SRNGNVLYSI RITLTLACPM DLKNFPMDVQ TCIMQLESFG YTMNDLIFEW QEQGAVQVAD GLTLPQFILK EEKDLRYCTK HYNTGKFTCI EARFHLERQM GY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Channel characteristics depend on the subunit composition, heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol. Reference: "Kinetic analysis of recombinant mammalian alpha(1) and alpha(1)beta glycine receptor channels." Mohammadi B., Krampfl K., Cetinkaya C., Moschref H., Grosskreutz J., Dengler R., Bufler J. Eur. Biophys. J. 32:529-536(2003). Function: nan