Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Cricetulus griseus, recombinant

Organism: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Source: E.coli. Expression Region: 2-333aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VKVGVNGFGR IGRLVTRAAF TSGKVEVVAI NDPFIDLNYM VYMFQYDSTH GKFKGTVKAE NGKLVINGKA ITIFQERDPA NIKWGDAGAE YVVESTGVFT TMEKAGAHLK GGAKRVIISA PSADAPMFVM GVNQDKYDNS LKIVSNASCT TNCLAPLAKV IHDNFGIVEG LMTTVHAITA TQKTVDGPSG KLWRDGRGAA QNIIPASTGA AKAVGKVIPE LNGKLTGMAF RVPTPNVSVV DLTCRLEKPA KYEDIKKVVK QASEGPLKGI LGYTEDQVVS CDFNSDSHSS TFDAGAGIAL NDNFVKLISW YDNEFGYSNR VVDLMAYMAS KE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs and suppresses their translation. Reference: "Nucleotide sequence of hamster glyceraldehyde-3-phosphate dehydrogenase mRNA." Vincent S., Fort P. Nucleic Acids Res. 18:3054-3054(1990). Function: nan