Glutamine synthetase(glnA1), Mycobacterium tuberculosis, recombinant

Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). Source: E.coli. Expression Region: 2-478aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-termina Myc-tagged. Target Protein Sequence: TEKTPDDVFK LAKDEKVEYV DVRFCDLPGI MQHFTIPASA FDKSVFDDGL AFDGSSIRGF QSIHESDMLL LPDPETARID PFRAAKTLNI NFFVHDPFTL EPYSRDPRNI ARKAENYLIS TGIADTAYFG AEAEFYIFDS VSFDSRANGS FYEVDAISGW WNTGAATEAD GSPNRGYKVR HKGGYFPVAP NDQYVDLRDK MLTNLINSGF ILEKGHHEVG SGGQAEINYQ FNSLLHAADD MQLYKYIIKN TAWQNGKTVT FMPKPLFGDN GSGMHCHQSL WKDGAPLMYD ETGYAGLSDT ARHYIGGLLH HAPSLLAFTN PTVNSYKRLV PGYEAPINLV YSQRNRSACV RIPITGSNPK AKRLEFRSPD SSGNPYLAFS AMLMAGLDGI KNKIEPQAPV DKDLYELPPE EAASIPQTPT QLSDVIDRLE ADHEYLTEGG VFTNDLIETW ISFKRENEIE PVNIRPHPYE FALYYDV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP. D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity. Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion. Involved in cell wall biosynthesis via the production of the major component poly-L-glutamine (PLG). PLG synthesis in the cell wall occurs only in nitrogen limiting conditions and on the contrary high nitrogen conditions inhibit PLG synthesis (Probable). Reference: n/a. Function: nan