Glutamate receptor ionotropic, NMDA 1 (GRIN1),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 19-559aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: RAACDPKIVN IGAVLSTRKH EQMFREAVNQ ANKRHGSWKI QLNATSVTHK PNAIQMALSV CEDLISSQVY AILVSHPPTP NDHFTPTPVS YTAGFYRIPV LGLTTRMSIY SDKSIHLSFL RTVPPYSHQS SVWFEMMRVY SWNHIILLVS DDHEGRAAQK RLETLLEERE SKAEKVLQFD PGTKNVTALL MEAKELEARV IILSASEDDA ATVYRAAAML NMTGSGYVWL VGEREISGNA LRYAPDGILG LQLINGKNES AHISDAVGVV AQAVHELLEK ENITDPPRGC VGNTNIWKTG PLFKRVLMSS KYADGVTGRV EFNEDGDRKF ANYSIMNLQN RKLVQVGIYN GTHVIPNDRK IIWPGGETEK PRGYQMSTRL KIVTIHQEPF VYVKPTLSDG TCKEEFTVNG DPVKKVICTG PNDTSPGSPR HTVPQCCYGF CIDLLIKLAR TMNFTYEVHL VADGKFGTQE RVNNSNKKEW NGMMGELLSG QADMIVAPLT INNERAQYIE FSKPFKYQGL TILVKKEIPR STLDSFMQPF Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. Sensitivity to glutamate and channel kinetics depend on the subunit composition. Reference: "Inducible expression of neuronal glutamate receptor channels in the NT2 human cell line." Younkin D.P., Tang C.-M., Hardy M., Reddy U.R., Shi Q.-Y., Pleasure S.J., Lee V.M.-Y., Pleasure D. Proc. Natl. Acad. Sci. U.S.A. 90:2174-2178(1993). Function: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+)