Glutamate carboxypeptidase 2 (FOLH1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 48-750aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EATNITPKHN MKAFLDELKA ENIKKFLYNF TQIPHLAGTE QNFQLAKQIQ SQWKEFGLDS VELAHYDVLL SYPNKTHPNY ISIINEDGNE IFNTSLFEPP PPGYENVSDI VPPFSAFSPQ GMPEGDLVYV NYARTEDFFK LERDMKINCS GKIVIARYGK VFRGNKVKNA QLAGAKGVIL YSDPADYFAP GVKSYPDGWN LPGGGVQRGN ILNLNGAGDP LTPGYPANEY AYRRGIAEAV GLPSIPVHPI GYYDAQKLLE KMGGSAPPDS SWRGSLKVPY NVGPGFTGNF STQKVKMHIH STNEVTRIYN VIGTLRGAVE PDRYVILGGH RDSWVFGGID PQSGAAVVHE IVRSFGTLKK EGWRPRRTIL FASWDAEEFG LLGSTEWAEE NSRLLQERGV AYINADSSIE GNYTLRVDCT PLMYSLVHNL TKELKSPDEG FEGKSLYESW TKKSPSPEFS GMPRISKLGS GNDFEVFFQR LGIASGRARY TKNWETNKFS GYPLYHSVYE TYELVEKFYD PMFKYHLTVA QVRGGMVFEL ANSIVLPFDC RDYAVVLRKY ADKIYSISMK HPQEMKTYSV SFDSLFSAVK NFTEIASKFS ERLQDFDKSN PIVLRMMNDQ LMFLERAFID PLGLPDRPFY RHVIYAPSSH NKYAGESFPG IYDALFDIES KVDPSKAWGE VKRQIYVAAF TVQAAAETLS EVA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. Reference: Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene.O'Keefe D.S., Su S.L., Bacich D.J., Horiguchi Y., Luo Y., Powell C.T., Zandvliet D., Russell P.J., Molloy P.L., Nowak N.J., Shows T.B., Mullins C., Vonder Haar R.A., Fair W.R., Heston W.D.W.Biochim. Biophys. Acta 1443:113-127(1998). Function: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression., FUNCTION