Glutamate carboxypeptidase 2 (FOLH1), partial, Biotinylated, swine, recombinant

Organism: Sus scrofa (Pig). Source: E.coli. Expression Region: 44-751aa. Protein Length: Partial. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: KSPNEAANIS PQHNVKKAFL DELKAENIKT FLYNFTRIPH LAGTEQNFQL AKQIQSQWKE FGLDSVELAH YDVLLSYPNK TRPNYISIID EDGNEIFNTS LFEPPPPGYE NVSDVVPPFS AFSPQGMPEG DLVYVNYART EDFFKLERDM KINCSGKILI ARYGKIFRGN KVKNAQLAGA KGIILYSDPA DYFAPGVQSY PDGWNLPGGG VQRGNILNLN GAGDPLTPGY PANEYAYRLQ IAEAVGLPRI PVHPIGYSDA QKLLEKMGGS APPDDSWKGS LHVPYNVGPG FTGNFSTQKV KMHIHSDNKV KRIYNVIGTL RGAVEPDRYV ILGGHRDSWV FGGIDPQSGA AVVHEIVRSF GKLKKEGWRP RRTVLFASWD AEEYGLFGST EWAEENSRIL QERGVAYINA DSSIEGNYTL RVDCTPLMYS LVYNLTKELQ SPDEGFEGKS LFESWNEKSP SPEFSGLPRI SKLGSGNDFE VFFQRLGIAS GRARYTKDWV TNKFSSYPLY HSVYETYELV EKFYDPTFKY HLAVAQVRGG IVFELANSVV RPFDCRDYAV VLRNYADKLY NISMNHPQEM KAYSVSFDSL FSAVKNFTEI ASNFSERVQD LDKNNPILLR IMNDQLMFLE RAFIVPLGLP DRAFYRHVIY APSSHNKYMG ESFPGIYDAL FDIENKVDPS KAWGEVKRQI SIAAFTVQAA AGTLREVA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. , Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. Reference: "Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II." Halsted C.H., Ling E.-H., Luthi-Carter R., Villanueva J.A., Gardner J.M., Coyle J.T. J. Biol. Chem. 273:20417-20424(1998). Function: nan