Glucose-6-phosphate isomerase (Gpi), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 2-558aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: AALTRNPEFQ KLLEWHRANS ANLKLRELFE ADPERFNHFS LNLNTNHGHI LLDYSKNLVN KEVLHMLVDL AKSRGVEAAR DNMFSGLKIN STEDRAVLHV ALRNRSNRSI MMDGKDVMPE VNKVLDKMKS FCQRVRSGDW KGYTGKAITD IINIGIGGSD LGPLMVTEAL KPYSKGGPRV WFVSNIDGTH IAKTLANLNP ESSLFIIASK TFTTQETITN AETAKEWFLQ AAKDPSAVAK HFVALSTNTD KVKEFGIDPK NMFEFWDWVG GRYSLWSAIG LSIALHVGFD HFEQLLSGAH WMDQHFMKTP LDKNAPVLLA LLGIWYINFY GCETHAMLPY DQYMHRFAAY FQQGDMESNG KYITKSGARV DYQTGPIVWG EPGTNGQHAF YQLIHQGTKM IPCDFLIPVQ TQHPIRNGLH HKILLANFLA QTEALMKGKS PEEARKELQA AGKSPEELEK LLPHKVFEGN RPTNSIVFTK LTPFILGALI AMYEHKIFVQ GIIWDINSFD QWGVELGKQL AKKIEPELDG SSAVTSHDSS TNGLIGFIKL QRDTKID. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion. Reference: "Effect of 6-phosphogluconate on phosphoglucose isomerase in rat brain in vitro and in vivo." Gaitonde M.K., Murray E., Cunningham V.J. J. Neurochem. 52:1348-1352(1989). Function: nan