Glucose-6-phosphate isomerase (GPI), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-554aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AALTRDPQFQ KLQQWYREHR SELNLRRLFD ANKDRFNHFS LTLNTNHGHI LVDYSKNLVT EDVMRMLVDL AKSRGVEAAR ERMFNGEKIN YTEGRAVLHV ALRNRSNTPI LVDGKDVMPE VNKVLDKMKS FCQRVRSGDW KGYTGKTITD VINIGIGGSD LGPLMVTEAL KPYSSGGPRV WYVSNIDGTH IAKTLAQLNP ESSLFIIASK TFTTQETITN AETAKEWFLQ AAKDPSAVAK HFVALSTNTT KVKEFGIDPQ NMFEFWDWVG GRYSLWSAIG LSIALHVGFD NFEQLLSGAH WMDQHFRTTP LEKNAPVLLA LLGIWYINCF GCETHAMLPY DQYLHRFAAY FQQGDMESNG KYITKSGTRV DHQTGPIVWG EPGTNGQHAF YQLIHQGTKM IPCDFLIPVQ TQHPIRKGLH HKILLANFLA QTEALMRGKS TEEARKELQA AGKSPEDLER LLPHKVFEGN RPTNSIVFTK LTPFMLGALV AMYEHKIFVQ GIIWDINSFD QWGVELGKQL AKKIEPELDG SAQVTSHDAS TNGLINFIKQ QRE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Reference: Gurney M.E.Cloning of a glucose phosphate isomerase/neuroleukin-like sperm antigen involved in sperm agglutination.Yakirevich E., Naot Y.Biol. Reprod. 62:1016-1023(2000). Function: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.