Gastric inhibitory polypeptide receptor(GIPR),partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 22-138aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: RAETGSKGQT AGELYQRWER YRRECQETLA AAEPPSGLAC NGSFDMYVCW DYAAPNATAR ASCPWYLPWH HHVAAGFVLR QCGSDGQWGL WRDHTQCENP EKNEAFLDQR LILERLQ. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GIPR at 2 µg/mL can bind Anti-GIPR recombinant antibody ( CSB-RA009438MA1HU). The EC50 is 16.18-18.70 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Reference: "The human GIP receptor: gene structure, functional expression of its cDNA, and chromosomal location." Usdin T.B., Gruber C., Modi W., Bonner T.I. Submitted to EMBL/GenBank/DDBJ databases (OCT-1995). Function: nan