Filamin A-interacting protein 1-like (FILIP1L), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-200aa. Protein Length: Partial of Isoform 3. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MVVDEQQRLT AQLTLQRQKI QELTTNAKET HTKLALAEAR VQEEEQKATR LEKELQTQTT KFHQDQDTIM AKLTNEDSQN RQLQQKLAAL SRQIDELEET NRSLRKAEEE LQDIKEKISK GEYGNAGIMA EVEELRKRVL DMEGKDEELI KMEEQCRDLN KRLERETLQS KDFKLEVEKL SKRIMALEKL EDAFNKSKQE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acts as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor-associated vasculature. Reference: "Functional characterization of filamin a interacting protein 1-like, a novel candidate for antivascular cancer therapy." Kwon M., Hanna E., Lorang D., He M., Quick J.S., Adem A., Stevenson C., Chung J.-Y., Hewitt S.M., Zudaire E., Esposito D., Cuttitta F., Libutti S.K. Cancer Res. 68:7332-7341(2008). Function: nan