Fibritin (wac), Enterobacteria phage T4, recombinant

Organism: Enterobacteria phage T4 (Bacteriophage T4). Source: E.coli. Expression Region: 2-487aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: TDIVLNDLPF VDGPPAEGQS RISWIKNGEE ILGADTQYGS EGSMNRPTVS VLRNVEVLDK NIGILKTSLE TANSDIKTIQ GILDVSGDIE ALAQIGINKK DISDLKTLTS EHTEILNGTN NTVDSILADI GPFNAEANSV YRTIRNDLLW IKRELGQYTG QDINGLPVVG NPSSGMKHRI INNTDVITSQ GIRLSELETK FIESDVGSLT IEVGNLREEL GPKPPSFSQN VYSRLNEIDT KQTTVESDIS AIKTSIGYPG NNSIITSVNT NTDNIASINL ELNQSGGIKQ RLTVIETSIG SDDIPSSIKG QIKDNTTSIE SLNGIVGENT SSGLRANVSW LNQIVGTDSS GGQPSPPGSL LNRVSTIETS VSGLNNAVQN LQVEIGNNSA GIKGQVVALN TLVNGTNPNG STVEERGLTN SIKANETNIA SVTQEVNTAK GNISSLQGDV QALQEAGYIP EAPRDGQAYV RKDGEWVFLS TFLSPA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Chaperone responsible for attachment of long tail fibers to virus particle. During phage assembly, twelve fibritin molecules attach to the phage neck via gp13: six molecules forming the collar and six molecules forming the whiskers. Reference: "gpwac of the T4-type bacteriophages: structure, function, and evolution of a segmented coiled-coil protein that controls viral infectivity."Letarov A., Manival X., Desplats C., Krisch H.M.J. Bacteriol. 187:1055-1066(2005). Function: Chaperone involved in tail fiber assembly and retraction. Acts as a chaperone helping to attach the long tail fibers to the virus during the assembly process. During phage assembly, twelve fibritin molecules attach to the phage neck via gp13