Fc receptor-like protein 3 (FCRL3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 18-573aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: GVAPKAVLLL NPPWSTAFKG EKVALICSSI SHSLAQGDTY WYHDEKLLKI KHDKIQITEP GNYQCKTRGS SLSDAVHVEF SPDWLILQAL HPVFEGDNVI LRCQGKDNKN THQKVYYKDG KQLPNSYNLE KITVNSVSRD NSKYHCTAYR KFYILDIEVT SKPLNIQVQE LFLHPVLRAS SSTPIEGSPM TLTCETQLSP QRPDVQLQFS LFRDSQTLGL GWSRSPRLQI PAMWTEDSGS YWCEVETVTH SIKKRSLRSQ IRVQRVPVSN VNLEIRPTGG QLIEGENMVL ICSVAQGSGT VTFSWHKEGR VRSLGRKTQR SLLAELHVLT VKESDAGRYY CAADNVHSPI LSTWIRVTVR IPVSHPVLTF RAPRAHTVVG DLLELHCESL RGSPPILYRF YHEDVTLGNS SAPSGGGASF NLSLTAEHSG NYSCDADNGL GAQHSHGVSL RVTVPVSRPV LTLRAPGAQA VVGDLLELHC ESLRGSFPIL YWFYHEDDTL GNISAHSGGG ASFNLSLTTE HSGNYSCEAD NGLGAQHSKV VTLNVTGTSR NRTGLT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Promotes TLR9-induced B-cell proliferation, activation and survival but inhibits antibody production and suppresses plasma cell differentiation. Enhances activation of NF-kappa-B and MAPK signaling pathways in TLR9 stimulated B-cells. Has inhibitory potentional on B-cell receptor (BCR)-mediated signaling, possibly through association with SH2 domain-containing phosphatases. Inhibits cell tyrosine phosphorylation, calcium mobilization and activation-induced cell death induced through BCR signaling. Regulatory T-cells expressing FCRL3 exhibit a memory phenotype, are relatively nonresponsive to antigenic stimulation in presence of IL2 and have reduced capacity to suppress the proliferation of effector T-cells. Reference: Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A. Submitted (OCT-2006). Function: nan