F-box only protein 3 (FBXO3), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 1-471aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MAAMETETAP LTLESLPTDP LLLILSFLDY RDLINCCYVS RRLSQLSSHD PLWRRHCKKY WLISEEEKTQ KNQCWKSLFI DTYSDVGRYI DHYAAIKKAW DDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT SYVKNVVSGG FPIIRDQIFR YVHDPECVAT TGDITVSVST SFLPELSSVH PPHYFFTYRI RIEMSKDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG EFPIISPGRV YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK IFNVAIPRFH MACPTFRVSI ARLEMGPDEY EEMEEEEEEE EEEDEDDDSA DMDESDEDDE EERRRRVFDV PIRRRRCSRL F. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Reference: "Complete sequencing and characterization of 21,243 full-length human cDNAs."Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004). Function: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.