Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1250-1599aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-B2M-JD-tagged and C-terminal Myc-tagged. Target Protein Sequence: IEEYLHLNDM KEAVQCVQEL ASPSLLFIFV RHGVESTLER SAIAREHMGQ LLHQLLCAGH LSTAQYYQGL YEILELAEDM EIDIPHVWLY LAELVTPILQ EGGVPMGELF REITKPLRPL GKAASLLLEI LGLLCKSMGP KKVGTLWREA GLSWKEFLPE GQDIGAFVAE QKVEYTLGEE SEAPGQRALP SEELNRQLEK LLKEGSSNQR VFDWIEANLS EQQIVSNTLV RALMTAVCYS AIIFETPLRV DVAVLKARAK LLQKYLCDEQ KELQALYALQ ALVVTLEQPP NLLRMFFDAL YDEDVVKEDA FYSWESSKDP AEQQGKGVAL KSVTAFFKWL REAEEESDHN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Reference: "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation." Imataka H., Gradi A., Sonenberg N. EMBO J. 17:7480-7489(1998). Function: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.