Eosinophil peroxidase (EPX), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 140-715aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: RCSDKYRTIT GRCNNKRRPL LGASNQALAR WLPAEYEDGL SLPFGWTPSR RRNGFLLPLV RAVSNQIVRF PNERLTSDRG RALMFMQWGQ FIDHDLDFSP ESPARVAFTA GVDCERTCAQ LPPCFPIKIP PNDPRIKNQR DCIPFFRSAP SCPQNKNRVR NQINALTSFV DASMVYGSEV SLSLRLRNRT NYLGLLAINQ RFQDNGRALL PFDNLHDDPC LLTNRSARIP CFLAGDTRST ETPKLAAMHT LFMREHNRLA TELRRLNPRW NGDKLYNEAR KIMGAMVQII TYRDFLPLVL GKARARRTLG HYRGYCSNVD PRVANVFTLA FRFGHTMLQP FMFRLDSQYR ASAPNSHVPL SSAFFASWRI VYEGGIDPIL RGLMATPAKL NRQDAMLVDE LRDRLFRQVR RIGLDLAALN MQRSRDHGLP GYNAWRRFCG LSQPRNLAQL SRVLKNQDLA RKFLNLYGTP DNIDIWIGAI AEPLLPGARV GPLLACLFEN QFRRARDGDR FWWQKRGVFT KRQRKALSRI SLSRIICDNT GITTVSRDIF RANIYPRGFV NCSRIPRLNL SAWRGT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Reference: "Human eosinophil peroxidase induces surface alteration, killing, and lysis of Mycobacterium tuberculosis." Borelli V., Vita F., Shankar S., Soranzo M.R., Banfi E., Scialino G., Brochetta C., Zabucchi G. Infect. Immun. 71:605-613(2003). Function: nan