Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial (Active), human, r

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 48-875aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: LEKQGSCRKK CFDASFRGLE NCRCDVACKD RGDCCWDFED TCVESTRIWM CNKFRCGETR LEASLCSCSD DCLQRKDCCA DYKSVCQGET SWLEENCDTA QQSQCPEGFD LPPVILFSMD GFRAEYLYTW DTLMPNINKL KTCGIHSKYM RAMYPTKTFP NHYTIVTGLY PESHGIIDNN MYDVNLNKNF SLSSKEQNNP AWWHGQPMWL TAMYQGLKAA TYFWPGSEVA INGSFPSIYM PYNGSVPFEE RISTLLKWLD LPKAERPRFY TMYFEEPDSS GHAGGPVSAR VIKALQVVDH AFGMLMEGLK QRNLHNCVNI ILLADHGMDQ TYCNKMEYMT DYFPRINFFY MYEGPAPRIR AHNIPHDFFS FNSEEIVRNL SCRKPDQHFK PYLTPDLPKR LHYAKNVRID KVHLFVDQQW LAVRSKSNTN CGGGNHGYNN EFRSMEAIFL AHGPSFKEKT EVEPFENIEV YNLMCDLLRI QPAPNNGTHG SLNHLLKVPF YEPSHAEEVS KFSVCGFANP LPTESLDCFC PHLQNSTQLE QVNQMLNLTQ EEITATVKVN LPFGRPRVLQ KNVDHCLLYH REYVSGFGKA MRMPMWSSYT VPQLGDTSPL PPTVPDCLRA DVRVPPSESQ KCSFYLADKN ITHGFLYPPA SNRTSDSQYD ALITSNLVPM YEEFRKMWDY FHSVLLIKHA TERNGVNVVS GPIFDYNYDG HFDAPDEITK HLANTDVPIP THYFVVLTSC KNKSHTPENC PGWLDVLPFI IPHRPTNVES CPEGKPEALW VEERFTAHIA RVRDVELLTG LDFYQDKVQP VSEILQLKTY LPTFETTI. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 µg/mL can bind anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 2.151-2.492 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity. Reference: n/a. Function: nan