Double-stranded RNA-specific adenosine deaminase (Adar), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 835-1170aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: SLGTGNRCVK GDSLSLKGET VNDCHAEIIS RRGFIRFLYS ELMKYNHHTA KNSIFELARG GEKLQIKKTV SFHLYISTAP CGDGALFDKS CSDRAVESTE SRHYPVFENP KQGKLRTKVE NGEGTIPVES SDIVPTWDGI RLGERLRTMS CSDKILRWNV LGLQGALLTH FLQPVYLKSV TLGYLFSQGH LTRAICCRVT RDGKAFEDGL RYPFIVNHPK VGRVSVYDSK RQSGKTKETS VNWCMADGYD LEILDGTRGT VDGPGKELSR VSKKNIFLQF KKLCSFRARR DLLQLSYGEA KKAARDYDLA KNYFKKSLRD MGYGNWISKP QEEKNF. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine, pre-mRNA splicing by altering splice site recognition sequences, RNA stability by changing sequences involved in nuclease recognition, genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication, and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells. Reference: n/a. Function: nan