DNA topoisomerase 1 (TOP1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 190-765aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: NKKKKPKKEE EQKWKWWEEE RYPEGIKWKF LEHKGPVFAP PYEPLPENVK FYYDGKVMKL SPKAEEVATF FAKMLDHEYT TKEIFRKNFF KDWRKEMTNE EKNIITNLSK CDFTQMSQYF KAQTEARKQM SKEEKLKIKE ENEKLLKEYG FCIMDNHKER IANFKIEPPG LFRGRGNHPK MGMLKRRIMP EDIIINCSKD AKVPSPPPGH KWKEVRHDNK VTWLVSWTEN IQGSIKYIML NPSSRIKGEK DWQKYETARR LKKCVDKIRN QYREDWKSKE MKVRQRAVAL YFIDKLALRA GNEKEEGETA DTVGCCSLRV EHINLHPELD GQEYVVEFDF LGKDSIRYYN KVPVEKRVFK NLQLFMENKQ PEDDLFDRLN TGILNKHLQD LMEGLTAKVF RTYNASITLQ QQLKELTAPD ENIPAKILSY NRANRAVAIL CNHQRAPPKT FEKSMMNLQT KIDAKKEQLA DARRDLKSAK ADAKVMKDAK TKKVVESKKK AVQRLEEQLM KLEVQATDRE ENKQIALGTS KLNYLDPRIT VAWCKKWGVP IEKIYNKTQR EKFAWAIDMA DEDYEF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. Reference: "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J. Nature 414:865-871(2001). Function: nan