DNA polymerase theta (POLQ), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1792-2590aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: GFKDNSPISD TSFSLQLSQD GLQLTPASSS SESLSIIDVA SDQNLFQTFI KEWRCKKRFS ISLACEKIRS LTSSKTATIG SRFKQASSPQ EIPIRDDGFP IKGCDDTLVV GLAVCWGGRD AYYFSLQKEQ KHSEISASLV PPSLDPSLTL KDRMWYLQSC LRKESDKECS VVIYDFIQSY KILLLSCGIS LEQSYEDPKV ACWLLDPDSQ EPTLHSIVTS FLPHELPLLE GMETSQGIQS LGLNAGSEHS GRYRASVESI LIFNSMNQLN SLLQKENLQD VFRKVEMPSQ YCLALLELNG IGFSTAECES QKHIMQAKLD AIETQAYQLA GHSFSFTSSD DIAEVLFLEL KLPPNREMKN QGSKKTLGST RRGIDNGRKL RLGRQFSTSK DVLNKLKALH PLPGLILEWR RITNAITKVV FPLQREKCLN PFLGMERIYP VSQSHTATGR ITFTEPNIQN VPRDFEIKMP TLVGESPPSQ AVGKGLLPMG RGKYKKGFSV NPRCQAQMEE RAADRGMPFS ISMRHAFVPF PGGSILAADY SQLELRILAH LSHDRRLIQV LNTGADVFRS IAAEWKMIEP ESVGDDLRQQ AKQICYGIIY GMGAKSLGEQ MGIKENDAAC YIDSFKSRYT GINQFMTETV KNCKRDGFVQ TILGRRRYLP GIKDNNPYRK AHAERQAINT IVQGSAADIV KIATVNIQKQ LETFHSTFKS HGHREGMLQS DQTGLSRKRK LQGMFCPIRG GFFILQLHDE LLYEVAEEDV VQVAQIVKNE MESAVKLSVK LKVKVKIGAS WGELKDFDV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA . MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation . POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends . POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions . The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ . The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates . Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining . Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs . Reference: "A role for DNA polymerase theta in promoting replication through oxidative DNA lesion, thymine glycol, in human cells." Yoon J.H., Roy Choudhury J., Park J., Prakash S., Prakash L. J. Biol. Chem. 289:13177-13185(2014). Function: nan