DNA-dependent protein kinase catalytic subunit (dnapkcs), partial, Dictyostelium discoideum, recombi

Organism: Dictyostelium discoideum (Slime mold). Source: E.coli. Expression Region: 3910-4250aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ELDYPFLIKG GEDLRLDQRI QQLFGIMNEI LKRDTACNKR SLNVTTYQVV PMTSKVGIIE WLNDTKPLRE ILEEQLAHQL KTPRSNVSIS KLESTKYHND WINSFAKYLK PNSPVGPLYQ QMFIHATRDD CAKKLEKQHS KVPENLLQNG IWSLSSSPES YLFIRNSFAR SLASFSVCSY VIGIGDRHLE NFLISQRDGR LIGIDFGHAF GTATQFLPIP ELMPFRLTRQ FTSFLRPLDS VGLLNHNMTY TLTALQNQKE ILLTTMDVFV KEPLLDWSKL ATRLVKEQGK HPKDTKNVWF PKQKIQIAKK KLELVNPAYI TLEELSGSVH SGLPYEKALS E. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1. Reference: "The dictyostelium kinome -- analysis of the protein kinases from a simple model organism." Goldberg J.M., Manning G., Liu A., Fey P., Pilcher K.E., Xu Y., Smith J.L. PLoS Genet. 2:E38-E38(2006). Function: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (By similarity). This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.