DNA-binding protein Ikaros (IKZF1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-477aa. Protein Length: Full Length of Isoform Ik7. Tag Info: N-terminal 6xHis-Avi-tagged. Target Protein Sequence: MDADEGQDMS QVSGKESPPV SDTPDEGDEP MPIPEDLSTT SGGQQSSKSD RVVASNVKVE TQSDEENGRA CEMNGEECAE DLRMLDASGE KMNGSHRDQG SSALSGVGGI RLPNGKLKCD ICGIICIGPN VLMVHKRSHT GERPFQCNQC GASFTQKGNL LRHIKLHSGE KPFKCHLCNY ACRRRDALTG HLRTHSVIKE ETNHSEMAED LCKIGSERSL VLDRLASNVA KRKSSMPQKF LGDKGLSDTP YDSSASYEKE NEMMKSHVMD QAINNAINYL GAESLRPLVQ TPPGGSEVVP VISPMYQLHK PLAEGTPRSN HSAQDSAVEN LLLLSKAKLV PSEREASPSN SCQDSTDTES NNEEQRSGLI YLTNHIAPHA RNGLSLKEEH RAYDLLRAAS ENSQDALRVV STSGEQMKVY KCEHCRVLFL DHVMYTIHMG CHGFRDPFEC NMCGYHSQDR YEFSSHITRG EHRFHMS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer of the CD3-delta gene. Repressor of the TDT gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF, in a single complex, to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells. Function is isoform-specific and is modulated by dominant-negative inactive isoforms. Reference: "The Ikaros gene, a central regulator of lymphoid differentiation, fuses to the BCL6 gene as a result of t(3,7)(q27,p12) translocation in a patient with diffuse large B-cell lymphoma." Hosokawa Y., Maeda Y., Ichinohasama R., Miura I., Taniwaki M., Seto M. Blood 95:2719-2721(2000). Function: Transcription regulator of hematopoietic cell differentiation