Dipeptidase 3 (Dpep3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 36-462aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: TCTLTTPSPS SAPTTPEASN ATTAPGIPND TATSGVTSDP RLREQALALM RDFPLVDGHN DLPLLLRELF QNQLQDVNLR NFTRGQTNLD RLRDGLVGAQ FWSAYIPCQT QDRDAVRLAL EQIDLIRRMC SAYPELELVT SADGLNNTQK LACLIGVEGG HSLDTSLAVL RSFYELGVRY LTLTFTCSTP WAESATKFRH HFYTNISGLT SFGEKVVEEM NRLGMMIDLS HASDTLVKQT LEVSQAPVIF SHSAARSVCD NLLNIPDDIL QLLKKNGGIV MVTLSMGVLQ CSLFANVSTV ADHFDHIRTV IGSEFIGIGG SYDGSGRFPQ GLEDVSTYPV LIEELLSRGW DERELQGVLR GNLLRVFRQV EQVREKSLGQ SPVEVKFPER QQSNTCHSHL LPQPQEDQHQ DTHLKVTKLP NILQRAS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine. The absence of activity may be due to the inability of serine (instead of aspartate found in DPEP1/2) at position 356 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. Does not hydrolyze leukotriene D4 (LTD4) into leukotriene E4 (LTE4). Does not hydrolyze the beta-lactam antibiotic imipenem. Reference: n/a. Function: nan