Dihydroorotate dehydrogenase (quinone), mitochondrial(Dhodh), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 1-395aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MAWRQLRKRA LDAVIILGGG GLLFTSYLTA TGDDHFYAEY LMPGLQRLLD PESAHRLAVR VTSLGLLPRA TFQDSDMLEV KVLGHKFRNP VGIAAGFDKN GEAVDGLYKL GFGFVEVGSV TPQPQEGNPR PRVFRLPEDQ AVINRYGFNS HGLSVVEHRL RARQQKQAQL TADGLPLGIN LGKNKTSEDA AADYAEGVRT LGPLADYLVV NVSSPNTAGL RSLQGKTELR HLLSKVLQER DALKGTRKPA VLVKIAPDLT AQDKEDIASV ARELGIDGLI VTNTTVSRPV GLQGALRSET GGLSGKPLRD LSTQTIREMY ALTQGRIPII GVGGVSSGQD ALEKIQAGAS LVQLYTALIF LGPPVVVRVK RELEALLKER GFTTVTDAIG ADHRR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway. Reference: "Suppression of experimental autoimmune neuritis by leflunomide." Korn T., Toyka K., Hartung H.P., Jung S. Brain 124:1791-1802(2001). Function: nan