D-dopachrome decarboxylase (Ddt), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 2-118aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal V5-tagged. Target Protein Sequence: PFVELETNLP ASRIPAGLEN RLCAATATIL DKPEDRVSVT IRPGMTLLMN KSTEPCAHLL ISSIGVVGTA EQNRSHSSSF FKFLTEELSL DQDRIIIRFF PLEPWQIGKK GTVMTFL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Reference: "Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase." Zhang M., Aman P., Grubb A., Panagopoulos I., Hindemith A., Rosengren E., Rorsman H. FEBS Lett. 373:203-206(1995). Function: nan