Cytosolic non-specific dipeptidase Protein (recombinant mouse)

Activity: Measured by its ability to cleave carnosine (ß-Ala-His) in a two step assay (mouse CNDP2 concentration 10 µg/ml). The specific activity is >40 pmoles/min/µg. Protein Construction: Recombinant Mouse Cytosolic non-specific dipeptidase is produced by our Mammalian expression system and the target gene encoding Met1-Asn475 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Asn 475. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 56-60 kDa. Protein function: Mouse cytosolic non-specific dipeptidase(CNDP2) is a cytoplasm protein which belongs to the peptidase M20A family. CNDP2 has 2 Isoform: Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 hydrolyzes a variety of dipeptides including L-carnosine and has a strong preference for Cys-Gly. It is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis. It may play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Protein function: Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis. [The UniProt Consortium]